Drs. Charlotte Van Driessche
Terrestrial Ecology Unit
Department of Biology
Ghent University
K.L. Ledeganckstraat 35
9000 Gent Belgium
E-mail: charlotte.vandriessche@ugent.be
Implementation of eDNA metabarcoding as an innovative tool for biomonitoring fish communities in lotic waters
Biomonitoring of fish fauna using environmental DNA methods (eDNA; sampling of shed DNA in water and detection of fish species via molecular techniques) could serve as a complementary technique to conventional, invasive, costly and time-consuming methods. Integration of eDNA metabarcoding (investigating the composition of entire fish communities) into existing regulatory monitoring frameworks, requires further insights and the development of practical guidelines.
Remaining challenges include critical aspects of sampling and quantification of eDNA in lotic waters, as well as potential technical bias in eDNA detection and abundance quantification, spatial and temporal shifts in eDNA patterns, and assessments of ecological status.
The main part of my research includes investigating the potential of eDNA methods for quality scoring of ecosystem health in rivers using the European Water Framework Directive and its implementation in Flanders as a practical starting point. By means of a large-scale sampling campaign, eDNA sampling will be compared to traditional electrofishing (TEF) to provide insights on spatio-temporal patterns of eDNA abundance in natural systems. Experimental validation and calibration of absolute measures for community abundance, optimization of metabarcoding workflow, as well as the transformation of eDNA metabarcoding measurements into discrete indices for biotic integrity, will bring about practical guidelines on the use of eDNA metabarcoding as a biomonitoring tool for ecosystem health.
Besides providing a roadmap towards effective implementation of eDNA methods for lotic biomonitoring of fish fauna, I am also working on eDNA methods for rapid detection and adequate eradication of invasive species, such as the American bullfrog, or the Asian weatherloach (Misgurnus bipartitus); as well as for the detection of native, yet elusive species (e.g. Misgurnus fossilis).